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Chromatography of Plants - Science Fair Projects

See the complete experiment on in deciduous and broadleaf evergreens
for chromatography of plants - Science Fair Projects
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What color pigments are hidden during the summer
and can be seen in the fall?



 

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Name: Megan
Grade: 2nd Year High School - Home School
Texas, USA
Science Category: Biology - Botany
Honorary Prize: Science Fair Enthusiast's Contest Fall 2008
Abstract

Objective of This Science Fair projects
The fall colors we see in trees are hidden throughout the summer by the pigment chlorophyll. But there are other pigments within the leaves that reveal themselves as the chlorophyll breaks down. Those pigments are xanthophylls (yellow), carotenoids (yellow, orange, red), and anthocyanins (red). I wanted to see if these pigments were present in both deciduous and broadleaf evergreens, and if so, which angiosperm—deciduous or broadleaf evergreens—would show a more intense pigmentation.

Hyothesis - Not submitted but required.

Materials Used & Experiment
I collected leaf samples throughout San Angelo, identifying their location using GPS. I then crushed them and used Isopropyl alcohol as my solvent to extract the pigment from the leaves. To separate the pigments I used a chromatography technique using coffee filters, drawing paper, and Viva™ paper towels.

Results
The results of my experiment showed that my hypothesis was partially correct. Both deciduous and broadleaf evergreens contained all the pigments, but I believed that it would be the deciduous that contained a higher intensity where as the results showed the broadleaf evergreens did.

Conclusion
This lead me to believe that it was possible that because did my sampling in the fall, there was less pigmentation in the deciduous because it was at the time of year chlorophyll was starting to break down and photosynthesis was slowing down as the deciduous trees were getting ready to lose their leaves and go dormant.

To see Megans complete project go to chromatography high school science fair projects.

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