Clean Biology Science Fair Projects

Clean biology science fair projects begins at microbiology antiseptic science fair projects.

PROCEDURES

1. Gather materials.
2. Isolate alpha streptococcus:
a. Swab mouth.
b. Inoculate plate.
c. Take inoculating loop, and streak plate for isolation.
d. Streak plate for growth.
e. Incubate overnight at 35*F at 7% CO2.
f. Take predominate colony.
3. Make suspension of organism:
a. Mix organism in saline solution in glass vial.
b. Insert in colorimeter and measure the density.
c. Continue adding bacteria to the vial until it has reached about 80% density.
d. Swab over Mueller Hinton plate with blood.
4. Innoculate plate with mouthwashes
a. Treat plate with 10 microliters of the first type of mouthwash to the right of the quadrant.
b. Treat plate with another dose of 10 microliters of the mouthwash to the left of the other 10 microliter dose.
c. Treat plate with 50 microliters of the mouthwash below the two 10 microliter dosages.
d. Add saline solution to the fourth quadrant for control.
e. Repeat steps 4a-4c with three mouthwashes and one control (saline) on 3 plate.
f. Incubate overnight at 35*F at 7% CO2.
4. Measure zones of death with a calipers where mouthwash was placed in millimeters.
5. Estimate the number of colonies within the death zones.
6. Record data.
7. All materials are put in the autoclave (heated at 250*F) to sterilize.

RESULTS

The original purpose of this experiment was to determine which mouthwash was most effective in killing alpha streptococcus.

The results of the experiment were that the least amount of bacteria colonies grew where Rembrandt or Scope had been. The most bacteria grew where Fluorigard, Biotene, and Long’s brand.

CONCLUSION

My hypothesis was that Listerene Antiseptic Mouthwash would kill the most alpha streptococcus.

The results indicate that this hypothesis should be rejected because Listerene did not kill an average of 70 bacteria colonies in Trial 1 and 2, while other mouthwashes killed all colonies.

Because of the results of this experiment, I wonder if the mouthwash would be effective in killing other types of mouth bacteria. I also wonder if toothpaste would work in killing bacteria.

If I were to conduct this experiment again, I would use a different type of bacteria. I would also use separate plates for each mouthwash, and use varied amounts of mouthwash (such as 5, 15, 25, 40, and 55 microliter dosages). I would also use more brands of mouthwash, and do more trials.