Disinfectants Microbiology Science Fair Projects with Bacteria

Disinfectants microbiology science fair projects begins at microbiology disinfectants science fair projects.

PROCEDURE

1. Gather all materials needed.
2. Add bacteria to culture flask.
3. Let stand for 24 hours.
4. Label 1 test tube C.
5. Label four more test tubes: 2a, 2b, 2c and 2d.
6. Label four more test tubes: 3a, 3b, 3c and 3d.
7. Label the last four test tubes: 4a, 4b, 4c and 4d.
8. Add 50ml of bacteria infested water to each test tube.
9. Set the control test tube aside which will have no antiseptic added.
10. Measure out 5ml of ammonia and add it to flask 2a.
11. Measure out 10ml of ammonia and add it to flask 2b.
12. Measure out 15ml of ammonia and add it to flask 2c.
13. Measure out 20ml of ammonia and add it to flask 2d.
14. Let flasks stand for two minutes.
15. Label the Petri dishes the same as the test tubes.
16. Add 1ml of each tube contents to each Petri dish labeled the same.
17. Measure 5ml of isopropyl alcohol and add it to flask 3a.
18. Measure 10ml of isopropyl alcohol and add it to flask 3b.
19. Measure 15ml of isopropyl alcohol and add it to flask 3c.
20. Measure 20ml of isopropyl alcohol and add it to flask 3d.
21. Let flasks stand for two minutes.
22. Add 1ml of each tube contents to each Petri dish labeled the same.
23. Measure 1ml of Lysol and add it to flask 4a.
24. Measure 5ml of Lysol and add it to flask 4b.
25. Measure 10ml of Lysol and add it to flask 4c.
26. Measure 20ml of Lysol and add it to flask 4d.
27. Let flasks stand for two minutes
28. Add 1ml of each tube contents to each Petri dish labeled the same.
29. Set incubator to 34 degrees Celsius.
30. Place all Petri dishes into the incubator.
31. Observe 72 hours from incubating and proceed with a colony count under a microscope.

RESULTS

The original purpose of this experiment was to determine which antiseptic works best against specific bacteria. The isopropyl alcohol did not even affect the bacteria at 5ml, disinfected 1/3 of them in the 10ml and totally killed them in the 15ml and 20ml. The ammonia proved to be less affective and barely disinfected at all. 5ml and 10ml didn’t work at all, 15 and 20ml did but almost too small to notice. Lysol was the most effective even after being deluded to the recommended concentration it disinfected the samples entirely in all of the amounts

CONCLUSION

My hypothesis is that isopropyl alcohol will work better than ammonia and Lysol.
The results indicate that this hypothesis should be rejected because the Lysol worked far better then the ammonia and the alcohol.
Because of the results of the experiment I wonder if the company that manufactures Lysol should create a product that hospitals could use on patients instead of isopropyl alcohol.
If I were to conduct this project again I would use antibacterial soap in my experiment and I also would have been more prepared for the scientific committee. I would of also of used two strands of bacteria to see if my results are more widely occurring. 3rd party credit