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Microbiology Disinfectant Science Fair Experiments with Bacteria

Experiment to find out which antiseptic works best against specific bacteria
for disinfectants microbiology science fair projects.
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Which disinfectant is best to use in your bathroom?
In your kitchen?

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PURPOSE

The purpose of this experiment was to determine which antiseptic works best against specific bacteria.

I became interested in this idea when I was on medication for a sickness and noticed how much the medication costs and its side effects. The information gained from this experiment might prevent people from getting sick if household items will kill sickness-causing bacteria around the house. It could prevent society from the wasting of time and money for doctor visits, buying prescription medicine and paying medical bills.

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Test bacteria on any surface with the Surface Microbes Science Fair Projects Kit: after hand sanitizer, hands, disinfectant.. for example. You can use antibiotics to test the bacteria too.

 

 

 

HYPOTHESIS

My hypothesis is that isopropyl alcohol will work better than ammonia and Lysol. I base my hypothesis on the fact that isopropyl alcohol is used in hospitals and doctors’ offices for antiseptic purposes before administrating injections.

EXPERIMENT DESIGN

The constants in this study were:
The same kind of bacteria was used in all tubes and Petri dishes.
The flasks were the same size and shape.
The same amount and type of agar was used in each tube and Petri dish.
The same method of separating bacteria was always used.
Measurements were made in the same manner.
Temperature, humidity and light were the same for all tubes and Petri dishes.
The same quantity of disinfectants was used in all tubes and Petri dishes.
The disinfectants touched bacteria the same amount of time.
The time of day of observation was almost the same.
The method of recording observations was consistent.

The manipulated variable was the type of antiseptic.

The responding variable was the amount of bacteria growth in the flacks during a 3-day period of time.

Measure the responding variable by recording the number of colonies in each Petri dish in each group. This will determine which antiseptic is most effective, how much of each of the original bacteria is eliminated and how fast does it reproduce after being exposed to the antiseptics.

MATERIALS

QUANTIY
ITEM DESCRIPTION
1
Unit of bacteria (Lactos Bacillus)
16
Petri dishes preloaded with agar
16
Test tubes
1
Bottle of isopropyl (rubbing) alcohol (70%)
1
Bottle of ammonia
1
Bottle of Lysol
1
Box of rubber gloves
1
Pencil
1
Pad of paper
2
Hours to conduct the experiment
1
Computer
1
Chair
1
Microscope

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