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Cleaning a Toothbrush Science Fair Projects

What's Growing on Your Toothbrush - Cleaning a Toothbrush Science Fair Projects
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Juniors in High School are grossed out
by what they find growing on the toothbrushes...

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Where Bacteria Grows

Bacteria can grow in almost any location on earth. It has been found deep underground and even in the ice sheets.

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Test bacteria on any surface with the Surface Microbes Science Fair Projects Kit: after hand sanitizer, hands, disinfectant.. for example. You can use antibiotics to test the bacteria too.

Anytime you want to test how many bacteria are On Top of a surface like a desk, skin, chicken, computer keyboard, bread dough, a hard piece of chocolate, cheese, inside of an animal's cheek, etc. then use the surface experimenter kit. The kit will let you calculate how many bacteria there are per unit surface area on the object. You can also test for e-coli, however, only the microbe water kit will let you distinguish e. coli from other coliforms and bacteria.

 

Objectives/Goals

My objective was to discover which method of cleaning one's toothbrush was most effective in eliminating pathogenic bacteria.

Methods/Materials

I chose to test the following methods: rinse with water, wash/rub with water, soak in mouthwash, soak in baking soda, zap in microwave, and no treatment/action after brush, which serves as the control. Before the start of each cleaning method, the subject toothbrush will be swiped across a #simulated mouth# bacteria bed for thirty seconds. After uniform treatment of all the toothbrushes, then the methods (the dependant variable) will be tested; samples will be then taken and transferred to nutrition agar petri dishes, which will be incubated and monitored for three days.

Results

Listerine mouthwash allowed no bacteria colonies to grow. The bacteria bed reached 883 colonies; Rinse with Water multiplied the bacteria colonies to an average of more than 1000 bacteria colonies. Wash/Rub with water averaged of 39 colonies. Soak in Baking Soda and Microwave 237.5 and 220.5, respectively. The control grew 2.5 bacteria colonies.

Conclusions/Discussion

The results of my investigation support my hypothesis that soaking a toothbrush in Listerine mouthwash is the most effective method at eliminating the number of bacteria colonies that can be grown on toothbrushes. Rinsing one#s toothbrush was actually more harmful than leaving the toothbrush alone The two #myth methods#, Soak in Baking Soda and Microwave, were proven to be un-effective because they produced over 200 bacteria colonies. 3rd party contributor


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