Germs Science Fair Projects at Supermarket Stores

Germs science fair projects abstract begins at clorex wipes science fair projects.

PROCEDURES

1. Go to Hospital Lab and obtain 18 sterile swabs.
2. Go to first destination.
3. Go to bathroom and locate outside doorknob.
4. Take out sterile swab
5. Collect Bacteria:
a. Rub halfway around doorknob as steadily as possible using even pressure for ten seconds using stopwatch.
b. Try to collect bacteria from entire surface.
6. Label Swab with three items.
a. Place bacteria were collected.
b. Inside or outside of bathroom door.
c. Before or after use of Clorox wipe.
7. Put swab back in carrying case.
8. Go inside bathroom.
9. Repeat steps four through seven.
10. Take out Clorox wipe.
11. Scrub down doorknob with Clorox wipe for 15 seconds using stopwatch.
12. Restart stopwatch
13. Let knob dry 60 seconds.
14. Repeat steps four through seven except on opposite side.
15. Repeat steps two through fourteen at all other destinations.
16. Go to Hospital Lab.
17. Put on Lab coat.
18. Transfer Bacteria from swabs to agar by rubbing in three different directions turning Petri dish 60 degrees each time.
19. Label Plates (as in step 6)
20. Repeat step 19 through 20 for all remaining swabs.
21. Incubate plates for 48 hours in incubator at 35° C.
22. Wash hands.
23. Count the number of colonies. If number is to large divide Petri dish into four sections, count each section and add together.
24. Record number of colonies.
25. Repeat steps 23 through 25 on all Petri dishes.
26. Wash hands
27. Sterilize Petri dishes by putting them in an autoclave. Incinerate swabs.

RESULTS

The original purpose of this experiment was to see the effect of Clorox wipes on the bacterial content on bathroom doorknobs.

The results of this experiment were that there was about 50% more bacteria on the inside than the outside door handle. The disinfectant wipe reduced it 100%. There was an equal amount of bacteria in restaurants and supermarkets and there was a little less that a quarter more in the homes.

CONCLUSION

The first original hypothesis was that there would be more bacteria on the inside door handle than the outside.

The results indicate that my 1st hypothesis should be accepted because there was over twice as much in the inside than the outside.

My second hypothesis was that there would be more bacteria in supermarket restrooms.

My 2nd hypothesis should be rejected because the supermarkets and restaurants had the same amount and the homes had approximately 25% more.

My third hypothesis was that the Clorox wipes would reduce the amount of bacteria significantly.

This hypothesis should be accepted because the Clorox wipes reduced the amount of bacteria 100% for all the trials.

My fourth hypothesis was that the Clorox wipes would not reduce the amount of bacteria entirely.

This hypothesis should be rejected because the Clorox wipes reduced the amount of bacteria 100%.

I realize there was a small flaw in this experiment. Maybe the bacteria weren’t removed by the Clorox disinfectant itself but instead by the process of rubbing the doorknob with the wipe. To solve this problem I could have tried using a spray instead of a wipe or towel. This could have solved my problem because I would have known if it was the disinfectant itself or the rubbing. I also could have solved the problem by rubbing with a wipe without disinfectant to see if rubbing alone removed the bacteria.

Because of the results of this experiment, I wonder how much bacteria would be reduced by high heat instead of applying disinfectant. For example, would a heat gun be an effective tool for killing bacteria on door knobs or other surfaces? 3rd party contributor